Optimization Procedure for Small Interfering RNA Transfection in a 384-Well Format
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چکیده
منابع مشابه
Optimal Electroporation Condition for Small Interfering RNA Transfection into MDA-MB-468 Cell Line
Background: Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...
متن کاملSmall interfering RNA; principles, applications and challenges--
Gene silencing using RNAi (RNA interference), has recently been used as a successful laboratory technique in determining the function and control of gene expression and provides a wide range of applications in molecular biology and gene therapy. RNAi is a method of suppressing gene expression. In this direction, a single-stranded RNA molecule of about 21–23 nucleotides, called siRNA (small inte...
متن کاملoptimal electroporation condition for small interfering rna transfection into mda-mb-468 cell line
background: electroporation is a valuable tool for small interfering rna (sirna) delivery into cells because it efficiently transforms a wide variety of cell types. since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect mda-mb 468 human breast cancer cell with sirna. ...
متن کاملAssay Assembly for Miniaturized Quantitative PCR in a 384-well Format using the Echo® Liquid Handler
Quantitative PCR (qPCR) has enabled a wide range of real-time applications including comparisons in gene expression, genotyping and SNP analysis. To ensure high data quality, the liquid handling employed in low-volume reactions must be exceptionally precise and accurate. This application note highlights the capabilities of the Echo 555 liquid handler to dispense into 384-well qPCR plates with t...
متن کاملOptimal Electroporation Condition for Small Interfering RNA Transfection into MDA-MB-468 Cell Line
BACKGROUND Electroporation is a valuable tool for small interfering RNA (siRNA) delivery into cells because it efficiently transforms a wide variety of cell types. Since electroporation condition for each cell type must be determined experimentally, this study presents an optimal electroporation strategy to reproducibly and efficiently transfect MDA-MB 468 human breast cancer cell with siRNA. ...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Screening
سال: 2007
ISSN: 1087-0571,1552-454X
DOI: 10.1177/1087057107300172